proteintech hif 1a Search Results


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Proteintech hif 1a
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Anti Hif 1a, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Proteintech mouse anti hypoxia inducible factor 1a
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Proteintech proteintech hif 1a
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Cell Signaling Technology Inc hif 1a
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Bio-Techne corporation hif-1 alpha antibody (h1alpha67)
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Santa Cruz Biotechnology hif 1a
Fig. 6. rhFGF21 inhibits proinflammatory activation of macrophage via downregulating <t>HIF-1a.</t> (A, B) Representative immunofluorescence and quantification of HIF-1a- F4/80 double positive macrophages in liver from mice in indicated groups (n = 5). DAPI (blue), HIF-1a (red), and F4/80 (green). White triangles indicate HIF-1a-F4/80 double positive macrophages. Scale bars: 50 lm. (C) The expression of HIF-1a in lysates of BMDMs in indicated groups, and normalized to b-actin (n = 4). (D, E) Representative immunofluorescence and quantification of iNOS-F4/80 double positive macrophages in liver from mice in indicated groups (n = 5). DAPI (blue), iNOS (red), and F4/80 (green). White arrows indicate iNOS-F4/80 double positive macrophages. Scale bars: 50 lm. (F) Elisa analysis for IL-1b, IL-6, and TNF-a in serum of mice in indicated groups (n = 5). (G) The Serum levels of ALT and AST from mice in indicated groups (n = 5). (H-J) Liver tissue samples from indicated groups were subjected to H&E staining, and infiltrated cells number around large vessel and small vessel was counted (n = 5). Scale bars: 50 lm. The region enclosed by the dashed lines represents the area infiltrated by inflammatory cells. (K, L) Representative image and quantification of hepatic MPO staining of mice in indicated groups (n = 5). Scale bars: 50 lm. Data are presented as mean ± SEM of each group. And one-way ANOVA test is using to assess the statistical differences in multiple groups. ns = no significant. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Hif 1a, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson hif-1a
Fig. 6. rhFGF21 inhibits proinflammatory activation of macrophage via downregulating <t>HIF-1a.</t> (A, B) Representative immunofluorescence and quantification of HIF-1a- F4/80 double positive macrophages in liver from mice in indicated groups (n = 5). DAPI (blue), HIF-1a (red), and F4/80 (green). White triangles indicate HIF-1a-F4/80 double positive macrophages. Scale bars: 50 lm. (C) The expression of HIF-1a in lysates of BMDMs in indicated groups, and normalized to b-actin (n = 4). (D, E) Representative immunofluorescence and quantification of iNOS-F4/80 double positive macrophages in liver from mice in indicated groups (n = 5). DAPI (blue), iNOS (red), and F4/80 (green). White arrows indicate iNOS-F4/80 double positive macrophages. Scale bars: 50 lm. (F) Elisa analysis for IL-1b, IL-6, and TNF-a in serum of mice in indicated groups (n = 5). (G) The Serum levels of ALT and AST from mice in indicated groups (n = 5). (H-J) Liver tissue samples from indicated groups were subjected to H&E staining, and infiltrated cells number around large vessel and small vessel was counted (n = 5). Scale bars: 50 lm. The region enclosed by the dashed lines represents the area infiltrated by inflammatory cells. (K, L) Representative image and quantification of hepatic MPO staining of mice in indicated groups (n = 5). Scale bars: 50 lm. Data are presented as mean ± SEM of each group. And one-way ANOVA test is using to assess the statistical differences in multiple groups. ns = no significant. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Hif 1a, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Cell Signaling Technology Inc rabbit anti hif 1a
Fig. 6. rhFGF21 inhibits proinflammatory activation of macrophage via downregulating <t>HIF-1a.</t> (A, B) Representative immunofluorescence and quantification of HIF-1a- F4/80 double positive macrophages in liver from mice in indicated groups (n = 5). DAPI (blue), HIF-1a (red), and F4/80 (green). White triangles indicate HIF-1a-F4/80 double positive macrophages. Scale bars: 50 lm. (C) The expression of HIF-1a in lysates of BMDMs in indicated groups, and normalized to b-actin (n = 4). (D, E) Representative immunofluorescence and quantification of iNOS-F4/80 double positive macrophages in liver from mice in indicated groups (n = 5). DAPI (blue), iNOS (red), and F4/80 (green). White arrows indicate iNOS-F4/80 double positive macrophages. Scale bars: 50 lm. (F) Elisa analysis for IL-1b, IL-6, and TNF-a in serum of mice in indicated groups (n = 5). (G) The Serum levels of ALT and AST from mice in indicated groups (n = 5). (H-J) Liver tissue samples from indicated groups were subjected to H&E staining, and infiltrated cells number around large vessel and small vessel was counted (n = 5). Scale bars: 50 lm. The region enclosed by the dashed lines represents the area infiltrated by inflammatory cells. (K, L) Representative image and quantification of hepatic MPO staining of mice in indicated groups (n = 5). Scale bars: 50 lm. Data are presented as mean ± SEM of each group. And one-way ANOVA test is using to assess the statistical differences in multiple groups. ns = no significant. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Rabbit Anti Hif 1a, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc hif-1a, cat no. 14179s
Fig. 6. rhFGF21 inhibits proinflammatory activation of macrophage via downregulating <t>HIF-1a.</t> (A, B) Representative immunofluorescence and quantification of HIF-1a- F4/80 double positive macrophages in liver from mice in indicated groups (n = 5). DAPI (blue), HIF-1a (red), and F4/80 (green). White triangles indicate HIF-1a-F4/80 double positive macrophages. Scale bars: 50 lm. (C) The expression of HIF-1a in lysates of BMDMs in indicated groups, and normalized to b-actin (n = 4). (D, E) Representative immunofluorescence and quantification of iNOS-F4/80 double positive macrophages in liver from mice in indicated groups (n = 5). DAPI (blue), iNOS (red), and F4/80 (green). White arrows indicate iNOS-F4/80 double positive macrophages. Scale bars: 50 lm. (F) Elisa analysis for IL-1b, IL-6, and TNF-a in serum of mice in indicated groups (n = 5). (G) The Serum levels of ALT and AST from mice in indicated groups (n = 5). (H-J) Liver tissue samples from indicated groups were subjected to H&E staining, and infiltrated cells number around large vessel and small vessel was counted (n = 5). Scale bars: 50 lm. The region enclosed by the dashed lines represents the area infiltrated by inflammatory cells. (K, L) Representative image and quantification of hepatic MPO staining of mice in indicated groups (n = 5). Scale bars: 50 lm. Data are presented as mean ± SEM of each group. And one-way ANOVA test is using to assess the statistical differences in multiple groups. ns = no significant. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Hif 1a, Cat No. 14179s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 6. rhFGF21 inhibits proinflammatory activation of macrophage via downregulating HIF-1a. (A, B) Representative immunofluorescence and quantification of HIF-1a- F4/80 double positive macrophages in liver from mice in indicated groups (n = 5). DAPI (blue), HIF-1a (red), and F4/80 (green). White triangles indicate HIF-1a-F4/80 double positive macrophages. Scale bars: 50 lm. (C) The expression of HIF-1a in lysates of BMDMs in indicated groups, and normalized to b-actin (n = 4). (D, E) Representative immunofluorescence and quantification of iNOS-F4/80 double positive macrophages in liver from mice in indicated groups (n = 5). DAPI (blue), iNOS (red), and F4/80 (green). White arrows indicate iNOS-F4/80 double positive macrophages. Scale bars: 50 lm. (F) Elisa analysis for IL-1b, IL-6, and TNF-a in serum of mice in indicated groups (n = 5). (G) The Serum levels of ALT and AST from mice in indicated groups (n = 5). (H-J) Liver tissue samples from indicated groups were subjected to H&E staining, and infiltrated cells number around large vessel and small vessel was counted (n = 5). Scale bars: 50 lm. The region enclosed by the dashed lines represents the area infiltrated by inflammatory cells. (K, L) Representative image and quantification of hepatic MPO staining of mice in indicated groups (n = 5). Scale bars: 50 lm. Data are presented as mean ± SEM of each group. And one-way ANOVA test is using to assess the statistical differences in multiple groups. ns = no significant. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Journal: Journal of advanced research

Article Title: FGF21 ameliorates septic liver injury by restraining proinflammatory macrophages activation through the autophagy/HIF-1α axis.

doi: 10.1016/j.jare.2024.04.004

Figure Lengend Snippet: Fig. 6. rhFGF21 inhibits proinflammatory activation of macrophage via downregulating HIF-1a. (A, B) Representative immunofluorescence and quantification of HIF-1a- F4/80 double positive macrophages in liver from mice in indicated groups (n = 5). DAPI (blue), HIF-1a (red), and F4/80 (green). White triangles indicate HIF-1a-F4/80 double positive macrophages. Scale bars: 50 lm. (C) The expression of HIF-1a in lysates of BMDMs in indicated groups, and normalized to b-actin (n = 4). (D, E) Representative immunofluorescence and quantification of iNOS-F4/80 double positive macrophages in liver from mice in indicated groups (n = 5). DAPI (blue), iNOS (red), and F4/80 (green). White arrows indicate iNOS-F4/80 double positive macrophages. Scale bars: 50 lm. (F) Elisa analysis for IL-1b, IL-6, and TNF-a in serum of mice in indicated groups (n = 5). (G) The Serum levels of ALT and AST from mice in indicated groups (n = 5). (H-J) Liver tissue samples from indicated groups were subjected to H&E staining, and infiltrated cells number around large vessel and small vessel was counted (n = 5). Scale bars: 50 lm. The region enclosed by the dashed lines represents the area infiltrated by inflammatory cells. (K, L) Representative image and quantification of hepatic MPO staining of mice in indicated groups (n = 5). Scale bars: 50 lm. Data are presented as mean ± SEM of each group. And one-way ANOVA test is using to assess the statistical differences in multiple groups. ns = no significant. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: And the primary antibodies included: MPO (Abcam, ab208670), F4/80 (Cell Signaling Technology, 30325), HIF-1a (Santa Cruz Biotechnology, sc-13515), iNOS (Santa Cruz Biotechnology, sc-7271), LC3 (Santa Cruz Biotechnology, sc376404), p62 (Santa Cruz Biotechnology, sc-48402) and albumin (Proteintech, 66051–1-Ig).

Techniques: Activation Assay, Expressing, Enzyme-linked Immunosorbent Assay, Staining